Detection of apoptosis by TUNEL assay

Kateryna Kyrylkova; Sergiy Kyryachenko; Mark Leid; Chrissa Kioussi

doi:10.1007/978-1-61779-860-3_5

Detection of apoptosis by TUNEL assay

Methods Mol Biol. 2012:887:41-7. doi: 10.1007/978-1-61779-860-3_5.

Authors

Kateryna Kyrylkova¹, Sergiy Kyryachenko, Mark Leid, Chrissa Kioussi

Affiliation

¹ Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA.

PMID: 22566045
DOI: 10.1007/978-1-61779-860-3_5

Abstract

Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.

MeSH terms

Animals
Apoptosis / physiology*
Embryo, Mammalian / cytology*
Fluorescent Antibody Technique
Frozen Sections / methods*
In Situ Nick-End Labeling / methods*
Mice