PDF DatasheetHuman AKT2 / Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant AKT2 / based upon sequence from Human
Host: QP5641 protein expressed in E. coli.
Tag: His-SUMO
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) AKT2 /, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP5641.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Asn2 – Glu481
Amino Acid Sequence: NEVSVIKEGW LHKRGEYIKT WRPRYFLLKS DGSFIGYKER PEAPDQTLPP LNNFSVAECQ LMKTERPRPN TFVIRCLQWT TVIERTFHVD SPDEREEWMR AIQMVANSLK QRAPGEDPMD YKCGSPSDSS TTEEMEVAVS KARAKVTMND FDYLKLLGKG TFGKVILVRE KATGRYYAMK ILRKEVIIAK DEVAHTVTES RVLQNTRHPF LTALKYAFQT HDRLCFVMEY ANGGELFFHL SRERVFTEER ARFYGAEIVS ALEYLHSRDV VYRDIKLENL MLDKDGHIKI TDFGLCKEGI SDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHERLFEL ILMEEIRFPR TLSPEAKSLL AGLLKKDPKQ RLGGGPSDAK EVMEHRFFLS INWQDVVQKK LLPPFKPQVT SEVDTRYFDD EFTAQSITIT PPDRYDSLGL LELDQRTHFP QFSYSASIRE
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human AKT2 / Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human AKT2 / Protein General Information | |
|---|---|
| Alternate Names | |
| AKT2 /, PRKBB, PKBBETA, RAC-BETA, HIHGHH, PKBB | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | AKT2 |
| Entrez Gene ID | 208 |
| Ensemble Gene ID | ENSG00000105221 |
| RefSeq Protein Accession(s) | NP_001317440.1 |
| RefSeq mRNA Accession(s) | NM_001330511.1, NM_001626.5, XM_011526614.1, XM_011526615.1, XM_011526616.1, XM_011526618.1, XM_011526619.1, XM_011526620.1, XM_017026470.1 |
| UniProt ID(s) | P31751 |
| UniGene ID(s) | Hs.631535 |
| HGNC ID(s) | HGNC:392 |
| COSMIC ID Link(s) | AKT2 |
| KEGG Gene ID(s) | hsa:208 |
| PharmGKB ID(s) | PA24685 |
| General Description of Recombinant Human AKT2 / Protein. | |
| AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of th, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at ‘Ser-50’ negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at ‘Ser-21’ and GSK3B at ‘Ser-9’, resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of ‘Ser-83’ decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at ‘Ser-939’ and ‘Thr-1462’, thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of mbers of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and Cytoplasmic domain localization. In particular, FOXO1 is phosphorylated at ‘Thr-24’, ‘Ser-256’ and ‘Ser-319’. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response elent binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates ‘Ser-454’ on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of ‘Ser-273’, resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on ‘Ser-318’, which results in increased PI3P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tpo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assbly and disassbly. May be involved in the regulation of the placental development.One of the few specific substrates of AKT2 identified recently is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 ses also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on ‘Ser-197’ during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. Phosphorylates CLK2 on ‘Thr-343’. | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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