WO2000015665A3 - A method of producing a functional immunoglobulin superfamily protein - Google Patents

A method of producing a functional immunoglobulin superfamily protein Download PDF

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Publication number
WO2000015665A3
WO2000015665A3 PCT/DK1999/000484 DK9900484W WO0015665A3 WO 2000015665 A3 WO2000015665 A3 WO 2000015665A3 DK 9900484 W DK9900484 W DK 9900484W WO 0015665 A3 WO0015665 A3 WO 0015665A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
functional
immunoglobulin superfamily
class
mhc class
Prior art date
Application number
PCT/DK1999/000484
Other languages
French (fr)
Other versions
WO2000015665A2 (en
Inventor
Lars Oestergaard Pedersen
Soeren Buus
Original Assignee
Lars Oestergaard Pedersen
Soeren Buus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lars Oestergaard Pedersen, Soeren Buus filed Critical Lars Oestergaard Pedersen
Priority to NZ511031A priority Critical patent/NZ511031A/en
Priority to CA002343313A priority patent/CA2343313A1/en
Priority to JP2000570203A priority patent/JP2002524104A/en
Priority to MXPA01002674A priority patent/MXPA01002674A/en
Priority to BR9914491-3A priority patent/BR9914491A/en
Priority to KR1020017003306A priority patent/KR20010075108A/en
Priority to EP99941420A priority patent/EP1114063A2/en
Priority to AU55047/99A priority patent/AU771239C/en
Publication of WO2000015665A2 publication Critical patent/WO2000015665A2/en
Publication of WO2000015665A3 publication Critical patent/WO2000015665A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators

Abstract

The present invention relates to a process of producing a functional immunoglobulin superfamily protein, which has at least one disulphide bond when functional, the process comprising the steps of providing a bacterial cell, where the gene is expressible, isolating the protein from the cell without reducing it and subjecting the isolated protein to a folding treatment. Preferably, the immunoglobulin superfamily protein is selected from the group consisting of Class I and Class II histocompatibility molecules and beta 2 microglobulin (beta2m). Other embodiments of the invention is a stable peptide free MHC protein obtainable by a process of the invention and a kit comprising a MHC class I heavy chain and a abeta2m allowing the recipient to produce and measure or detect a functional MHC class I protein to which a peptide, which is capabe of binding to said MHC class I protein, can be added leading to the generation of a functional MHC class I protein.
PCT/DK1999/000484 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein WO2000015665A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
NZ511031A NZ511031A (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein
CA002343313A CA2343313A1 (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein
JP2000570203A JP2002524104A (en) 1998-09-14 1999-09-14 Methods for producing functionalized immunoglobulin superfamily proteins
MXPA01002674A MXPA01002674A (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein.
BR9914491-3A BR9914491A (en) 1998-09-14 1999-09-14 Process for producing a functional immunoglobulin superfamily protein
KR1020017003306A KR20010075108A (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein
EP99941420A EP1114063A2 (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein
AU55047/99A AU771239C (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199801155 1998-09-14
DKPA199801155 1998-09-14

Publications (2)

Publication Number Publication Date
WO2000015665A2 WO2000015665A2 (en) 2000-03-23
WO2000015665A3 true WO2000015665A3 (en) 2000-08-17

Family

ID=8101632

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1999/000484 WO2000015665A2 (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein

Country Status (10)

Country Link
EP (1) EP1114063A2 (en)
JP (1) JP2002524104A (en)
KR (1) KR20010075108A (en)
CN (1) CN1326466A (en)
AU (1) AU771239C (en)
BR (1) BR9914491A (en)
CA (1) CA2343313A1 (en)
MX (1) MXPA01002674A (en)
NZ (1) NZ511031A (en)
WO (1) WO2000015665A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8969042B2 (en) 2004-08-19 2015-03-03 Biogen Idec Ma Inc. Refolding transforming growth factor beta family proteins
US9138461B2 (en) 2007-05-01 2015-09-22 Biogen Ma Inc. Compositions and methods for increasing vascularization
US9404916B2 (en) 2008-09-20 2016-08-02 University College Cardiff Consultants Limited Use of a protein kinase inhibitor to detect immune cells, such as T cells

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AU2002240818C1 (en) 2001-03-14 2008-11-06 Agilent Technologies, Inc. MHC molecule constructs and their uses for diagnosis and therapy
WO2003097669A2 (en) * 2002-05-17 2003-11-27 Københavns Universitet Method for purifying denatured proteins having a desired disulfide bond configuration
US20040072262A1 (en) * 2002-10-11 2004-04-15 Montero-Julian Felix A. Methods and systems for detecting MHC class I binding peptides
WO2004072112A2 (en) * 2003-02-11 2004-08-26 Københavns Universitet A method for formulating mhc-like molecules for long time storage
EP1706720A4 (en) * 2003-10-31 2007-02-28 Vitatex Inc Blood test prototypes and methods for the detection of circulating tumor and endothelial cells
WO2006009838A2 (en) 2004-06-17 2006-01-26 Beckman Coulter, Inc. Mycobacterium tuberculosis epitopes and methods of use thereof
TWI501774B (en) 2006-02-27 2015-10-01 Biogen Idec Inc Treatments for neurological disorders
CL2007002225A1 (en) * 2006-08-03 2008-04-18 Astrazeneca Ab SPECIFIC UNION AGENT FOR A RECEIVER OF THE GROWTH FACTOR DERIVED FROM PLATES (PDGFR-ALFA); NUCLEIC ACID MOLECULA THAT CODIFIES IT; VECTOR AND CELL GUESTS THAT UNDERSTAND IT; CONJUGADO UNDERSTANDING THE AGENT; AND USE OF THE AGENT OF A
EP2361930A3 (en) 2007-03-26 2011-10-26 Dako Denmark A/S Multimers of MHC-peptide complexes and uses thereof in Borrelia infectious diseases
WO2009003493A2 (en) 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
EP2188302B1 (en) 2007-07-09 2017-11-01 Genentech, Inc. Prevention of disulfide bond reduction during recombinant production of polypeptides
WO2009039854A2 (en) 2007-09-27 2009-04-02 Dako Denmark A/S Mhc multimers in tuberculosis diagnostics, vaccine and therapeutics
GB2453589A (en) 2007-10-12 2009-04-15 King S College London Protease inhibition
US10968269B1 (en) 2008-02-28 2021-04-06 Agilent Technologies, Inc. MHC multimers in borrelia diagnostics and disease
WO2010009735A2 (en) 2008-07-23 2010-01-28 Dako Denmark A/S Combinatorial analysis and repair
US10369204B2 (en) 2008-10-02 2019-08-06 Dako Denmark A/S Molecular vaccines for infectious disease
CN103364558A (en) * 2013-07-17 2013-10-23 江阴泽成生物技术有限公司 Human tumor marker carcinoembryonic antigen (CEA) magnetic particle chemiluminiscence immunoassay kit and detection method
CN109402036A (en) * 2018-11-07 2019-03-01 河北科技师范学院 The application of ox source Salmonella enteritidis trxB gene delection

Citations (5)

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WO1993022332A2 (en) * 1992-04-24 1993-11-11 Board Of Regents, The University Of Texas System Recombinant production of immunoglobulin-like domains in prokaryotic cells
WO1994006813A1 (en) * 1992-09-15 1994-03-31 The Rockefeller University Major histocompatibility complex molecules and modifications thereof
WO1995011702A1 (en) * 1993-10-25 1995-05-04 Anergen, Inc. Prokaryotic expression of mhc proteins
US5529921A (en) * 1992-02-19 1996-06-25 Scripps Research Institute In vitro activation of cytotoxic t-cells using insect cells expressing human class I MHC and β2-microglobulin
WO1997044667A2 (en) * 1996-05-21 1997-11-27 Institut Pasteur Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells

Patent Citations (5)

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US5529921A (en) * 1992-02-19 1996-06-25 Scripps Research Institute In vitro activation of cytotoxic t-cells using insect cells expressing human class I MHC and β2-microglobulin
WO1993022332A2 (en) * 1992-04-24 1993-11-11 Board Of Regents, The University Of Texas System Recombinant production of immunoglobulin-like domains in prokaryotic cells
WO1994006813A1 (en) * 1992-09-15 1994-03-31 The Rockefeller University Major histocompatibility complex molecules and modifications thereof
WO1995011702A1 (en) * 1993-10-25 1995-05-04 Anergen, Inc. Prokaryotic expression of mhc proteins
WO1997044667A2 (en) * 1996-05-21 1997-11-27 Institut Pasteur Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells

Non-Patent Citations (6)

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Title
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; MOLLOY P E ET AL: "Production of soluble single-chain T-cell receptor fragments in Escherichia coli trxB mutants.", XP002131051, retrieved from STN Database accession no. 1998346783 *
GARBOCZI D N ET AL: "HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (1992 APR 15) 89 (8) 3429-33., XP002131059 *
MOLECULAR IMMUNOLOGY, (1998 FEB) 35 (2) 73-81. *
PARKER K C ET AL: "An HLA-A2/beta 2-microglobulin/peptide complex assembled from subunits expressed separately in Escherichia coli.", MOLECULAR IMMUNOLOGY, (1992 MAR) 29 (3) 371-8., XP000876800 *
PLAKSIN D ET AL: "A T cell receptor V alpha domain expressed in bacteria: does it dimerize in solution?.", JOURNAL OF EXPERIMENTAL MEDICINE, (1996 OCT 1) 184 (4) 1251-8., XP000876791 *
STEVENS JAMES ET AL: "Efficient generation of major histocompatibility complex class I-peptide complexes using synthetic peptide libraries.", JOURNAL OF BIOLOGICAL CHEMISTRY JAN. 30, 1998, vol. 273, no. 5, 30 January 1998 (1998-01-30), pages 2874 - 2884, XP002136475, ISSN: 0021-9258 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8969042B2 (en) 2004-08-19 2015-03-03 Biogen Idec Ma Inc. Refolding transforming growth factor beta family proteins
US9138461B2 (en) 2007-05-01 2015-09-22 Biogen Ma Inc. Compositions and methods for increasing vascularization
US9404916B2 (en) 2008-09-20 2016-08-02 University College Cardiff Consultants Limited Use of a protein kinase inhibitor to detect immune cells, such as T cells

Also Published As

Publication number Publication date
WO2000015665A2 (en) 2000-03-23
NZ511031A (en) 2003-11-28
EP1114063A2 (en) 2001-07-11
AU5504799A (en) 2000-04-03
BR9914491A (en) 2002-01-15
CN1326466A (en) 2001-12-12
CA2343313A1 (en) 2000-03-23
JP2002524104A (en) 2002-08-06
AU771239B2 (en) 2004-03-18
AU771239C (en) 2004-10-14
MXPA01002674A (en) 2002-06-04
KR20010075108A (en) 2001-08-09

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